mouse il 12 23 p40  (R&D Systems)

Journal: Nature communications

Article Title: p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome.

doi: 10.1038/s41467-023-44390-w

Figure Lengend Snippet: Fig. 5 | p53 deficiency enhances IL12-p40 expression in mice post-TBI. a Left: images showing cytokine levels in mouse serum at 3 days post 12 Gy TBI deter- mined by Proteome Profiler Mouse XL Cytokine Array. Right: quantification of serum IL12-p40 levels. Each array represents a pool of 3 mice with the same treatment. Dots in yellow, blue, and red boxes represent IL12-p40, Flt3l and EGF, respectively. b The protein levels of IL12-p40 in mouse serum at 3 days post-TBI determined by ELISA assays. n = 4 mice/group. c The mRNA levels of IL12b nor- malized with the actin gene (left) and protein levels of IL12-p40 (right) in the SI of mice at 3 days post-TBI determined by qPCR and ELISA assays, respectively. n = 3 and 4 mice/group for left and right panels, respectively. The IL12b mRNA levels in p53+/+ mice without TBI are designated as 1. d The mRNA levels of IL12b were

Article Snippet: The protein levels of LIF and IL12-p40 were determined using a LIF Duoset kit (Cata#: DY449, R&D) and an IL12-p40 Duoset kit (Cata#: DY2398-05, R&D), respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Nature communications

Article Title: p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome.

doi: 10.1038/s41467-023-44390-w

Figure Lengend Snippet: Fig. 6 | p53 protects mice from radiation-induced damage by inhibiting IL12- p40 expression. a Schematic diagram of experimental procedures of aIL12-p40 antibody administration (i.p., 200 μg/mouse, once a day for 4 days from 2 days before till 2 days post-TBI). b–g aIL12-p40 antibody preferentially inhibited the inflammatory immune response in p53−/−mice post-TBI. b MFI of MHC-II on IECs from mice at 3 days post-TBI. n = 5 mice/group. c The number of CD25+ activated CD4+ (left) and CD8+ (right) T cells in LPs of mice at 3 days post-TBI. n = 5 mice/ group. d The mRNA levels of TNFα normalized with the actin gene in the SI of mice at 3 days post-TBI. n = 5 mice/group. The TNFα mRNA levels in p53+/+ mice post-TBI are designated as 1. e, f aIL12-p40 antibody protected ISCs from TBI-induced

Article Snippet: The protein levels of LIF and IL12-p40 were determined using a LIF Duoset kit (Cata#: DY449, R&D) and an IL12-p40 Duoset kit (Cata#: DY2398-05, R&D), respectively.

Techniques: Expressing